Instruments
 

Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer with a Dionex U-3000 Rapid Separation nano LC system

The Lumos was installed in January 2018 and is interfaced with a tandem Dionex nano RSLC system. This instrument combines the best of quadrupole, ion trap and Orbitrap mass analysis in a revolutionary Tribrid architecture to provide unprecedented depth of analysis. This instrument is particularly well-suited for use in isobaric-labeling quantitative proteomic studies because it is capable of MS3-based measurement of reporter ion intensities. Here, synchronous precursor selection methods enable simultaneous isolation of up to 20 MS2 fragment ions for further fragmentation to generate MS3 reporter ions that are detected using the high resolution Orbitrap. This dramatically increases reporter ion selectivity, providing substantial improvements in accuracy compared to MS2-based methods. The instrument typically is operated in the nanoLC-mode by Facility staff.


Thermo Q Exactive HF mass spectrometer with a Dionex U-3000 Rapid Separation nano LC system

This instrument was installed in December 2014 and is interfaced with a tandem Dionex nano RSLC system. The mass spectrometer uses a quadruple for precursor ion selection with HCD fragmentation and high-resolution, Orbitrap detection. Multiplex scanning (MSX) further increases duty cycle for targeted MS/MS. This instrument has a broad range of qualitative and quantitative applications and is operated by Facility staff.


Thermo LTQ-Orbitrap-Velos Pro ETD mass spectrometer with a Dionex U-3000 Rapid Separation nano LC system

The LTQ-Orbitrap-Velos Pro was installed in 2009 and is a high-resolution linear ion-trap mass spectrometer. This instrument is primarily used for H-D exchange experiments and small molecule analysis.


ABI-MDS SCIEX 4800 MALDI-TOF/TOF mass spectrometer

The 4800 can be used to perform both MS and MS/MS experiments on a wide variety of compounds including proteins, peptides, polymers, and organic compounds. This instrument can be either operated by the facility on behalf of clients or by users themselves. Investigators wishing to operate the instrument directly will receive initial training by facility staff and subsequent guidance as needed. Frequent users are advised to join our 4800 User's Group for discounted pricing.


HPLCs

LC Packings Ultimate LC equipped with a UV-VIS detector and Probot. This instrument typically is used by Facility staff for LC-MALDI or for initial prefractionation steps in MUDPIT experiments.

Gilson HPLC equipped with a UV/VIS detector. This system is operated either by Facility staff on behalf of clients or directly by users for separation of proteins, peptides, glycans, nucleotides and other compounds of interest.


 
 
Biological Mass Spectrometry Facility
Robert wood Johnson medical school
and
Rutgers, the state university of new jersey